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DNase I protection assay.
Note: DNA was released by adding heparin (100 U/μg DNA) to the complexes after treatment with DNase I (1 U/μg DNA).
Abbreviations: LPCs, polyethyleneimine 800-DNA complexes; SPCs, polyethyleneimine 800 conjugated poly(styrene-co-maleic anhydride)-DNA complexes; HPCs, polyethyleneimine 25,000-DNA complexes.
