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. 2012 Sep 14;7:4961–4972. doi: 10.2147/IJN.S32069

Figure 9.

Figure 9

(A) Quantitative analysis of cellular uptake of pDNA, PEI 800-DNA complexes, PEI 25,000-DNA complexes, and SP-DNA complexes in MCF-7 cells (A1) and MCF-7/ADR cells (A2). (B) Flow cytometry images of control cells (a, black), and cells treated with pDNA (b, green), PEI 800-DNA complexes (c, pink), PEI 25,000-DNA complexes (d, turquoise), SP-DNA complexes (e, red) in MCF-7 cells (B1), and MCF-7/ADR cells (B2). (C) Confocal microscopic images of MCF-7 cells and MCF-7/ADR cells after treatment with pDNA, PEI 800-DNA complexes, PEI 25,000-DNA complexes, and SP-DNA complexes for 2 hours.

Note: Nuclei (blue), lysosomes (red), and pDNA (green) were stained with Hoechst, LysoTracker red, and YOYO-1, respectively.

Abbreviations: LPCs, polyethyleneimine 800-DNA complexes; SPCs, polyethyleneimine 800 conjugated poly(styrene-co-maleic anhydride)-DNA complexes; HPCs, polyethyleneimine 25,000-DNA complexes; SP, polyethyleneimine 800 conjugated poly(styrene-co-maleic anhydride); PEI, polyethyleneimine.