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. 2012 Sep 19;7(9):e45332. doi: 10.1371/journal.pone.0045332

Figure 6. Relationship between apoptosis and the activity of Hedgehog pathway in HT-29 cells.

Figure 6

Flow cytometric demonstration of HT-29 apoptosis after 24 hours of exposure to different stimuli, as assessed by annexin-V/7-AAD. Left lower quadrant indicates double negative cells while right lower quadrant indicates annexin-V-positive cells (A). Treatment with Shh, purmorphamine or butyrate significantly abrogates the anti-CD95 induced apoptosis (*P<0.02), which is partially restored by the addition of cyclopamine (B). Data are expressed as the mean ± SEM of 3 independent experiments.