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. 2012 Sep 19;7(9):e45411. doi: 10.1371/journal.pone.0045411

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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GPLs from each M. avium spp. isolate were purified from total lipid extracts. Total GPL profiles were examined by TLC using chloroform:methanol:water (30∶8:1, v/v/v) as the solvent system and α-naphthol as the developer. Serovars were assigned based on a previous publication [25]. Serovar-specific polar GPLs (ssGPLs); serovar non-specific apolar GPLs (nsGPLs). Representative experiment of N = 3.