Figure 4. Detection of ManLAM in the cell wall of M. avium spp. isolates. A.

PAS-stained 15% SDS-polyacrylamide gel of whole cell lysates normalized by protein content (representative of N = 3). M.tb H₃₇R_v ManLAM was used as a control and clinical isolate MAC 104 as the reference standard; lane MM represents a pre-stained protein ladder. B. Western blot using anti-LAM CS-35 mAb (representative of N = 3).