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. 2012 Sep 19;7(9):e45730. doi: 10.1371/journal.pone.0045730

Figure 5. Co-expression of truncated NSs in RVFV-infected cells.

Figure 5

293 cells were mock-infected or infected with rMP12-NSs-Flag at an moi of 3, and mock-transfected or immediately transfected with in vitro transcribed RNA encoding CAT (control) or NSs with indicated truncations. Cells were collected at 16 hpi, and PKR (anti-PKR antibody), NSs (anti-RVFV antibody), NSs-Flag (anti-Flag antibody), CAT-myc (anti-myc antibody) and β-actin (anti-actin antibody) were detected by Western blot. (B) A549 cells were mock-infected or infected with rMP12-NSs-Flag at an moi of 3, and mock-transfected or immediately transfected with in vitro transcribed RNA encoding CAT or NSs with indicated truncations. Total RNA was extracted at 7 hpi, and IFN-β mRNA, ISG56 mRNA and RVFV anti-viral-sense S-RNA/N mRNA were detected by Northern blot with specific RNA probe. Representative data from at least 3 independent experiments are shown.