Figure 4. Fluid shear stress-induced cytoskeletal stresses in the presence of MSC inhibitors Gd³⁺ and GsMTx4.

a,b: Application of 100 µM Gd³⁺ (solid squares) or 5 µM GsMTx4 (unfilled squares) inhibited the dynamic changes in actinin stresses under flow, showing only small variations. c,d: Fluorescence images (CFP channel) and FRET ratio at various times in (a) (solid square), showing that shear stress did not cause significant redistribution of cytoskeleton, and cell showed active locomotion. The scale bar represents 10 µm.