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. 2012 Sep 19;7(9):e45133. doi: 10.1371/journal.pone.0045133

Figure 2. The miR-106b-93-25 cluster promoted EMC cell growth.

Figure 2

(a)At indicated times post-transfection, MTT assays were used to compare the effects of miR-106b, miR-93, miR-25 and their inhibitors. miR-106b, miR-93, miR-25 promoted the growth of ECC-1 cells, while their inhibitors significantly decreased proliferation in ECC-1 cells. Values represent the means ± SD from triplicate determinations. (b) Effects of miR-106b, miR-93 or miR-25 on the cell cycle. FACS analysis was performed on cells transfected with either miRNA or miRNA inhibitors. miR-106b and miR-93 gain of function led to an increase in cells in S-phase, while the G1-phase population was increased in the cells transfected with their inhibitors. miR-25 gain of function had no effects on the cell cycle. (c) Effects of miR-106b or miR-93 or miR-25 on apoptosis. FACS analysis was performed on cells transfected with either miRNA or miRNA inhibitors. miR-25 gain of function decreased apoptosis of cells. Apoptosis increased significantly in cells transfected with the miR-25 inhibitor. miR-106b and miR-93 had no effect on the apoptosis levels of the cells.