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. 2012 Sep 19;7(9):e45133. doi: 10.1371/journal.pone.0045133

Figure 4. TSA downregulated the miR-106b-93-25 cluster by downregulating MYC.

Figure 4

(a) The mRNA and protein levels of MYC was downregulated in ECC-1 and HEC-1A cells treated with TSA for 24 h. (b) Depletion of MYC inhibited the expression of miR-106-93-25, as well as its host gene MCM7, and promoted the expression of P21 and BIM mRNA and protein expressions in ECC-1 and HEC-1A cells. (c) Overexpression of MYC upregulated expressions of miR-106b-93-25 and MCM7, and downregulated P21 and BIM mRNA expressions. (d) P21 mRNA and protein levels were upregulated in ECC-1 cells treated with TSA for 24 h, while it was partially neutralized by miR-106b (left). BIM mRNA was upregulated in ECC-1 cells treated with TSA for 24 h, while it was not neutralized by miR-25, but its protein level was neutralized in miR-25 overexpressing ECC-1 cells. **, P<0.01; *, P<0.05; ns, not significant; paired t-test.