Figure 7. Effect of GRP78 coating concentration on the binding of PAT-SM6 to immobilised GRP78.

The primary antibodies used were: A. PAT-SM6; B. polyclonal anti-GRP78; C. PAT-SM6-hex and D. PAT-SM6 IgG. The GRP78 coating concentrations were 30 µg/mL (closed circles), 15 µg/mL (open inverted triangles), 7.5 µg/mL (closed squares), 3.75 µg/mL (open diamonds), 1.88 µg/mL (closed triangles), 0 µg/mL (open circles). The solid lines are best-fit lines calculated for a simple binding isotherm.