Figure 9. Competition ELISA data obtained using soluble GRP78 as a competitor.

A. The concentration of GRP78 used to coat the wells was 7.5 µg/mL. The primary antibodies used were PAT-SM6 (5 µg/ml, closed circles) and polyclonal anti-GRP78 (open circles). Results are also presented for control experiments omitting GRP78 coat using as primary antibodies PAT-SM6 (5 mg/ml, closed triangles) or anti-GRP78 (open squares). B: The binding of PAT-SM6 (5 µg/ml) to immobilized GRP78 at different GRP78 coating concentrations in the absence (black bars) or presence (grey bars) of soluble GRP78 (4 mg/ml). C: Soluble GRP78-induced inhibition of PAT-SM6 binding to immobilised GRP78 at different GRP78 coating concentrations. The concentrations of PAT-SM6 and soluble GRP78 used were 5 µg/ml and 4 mg/ml respectively.