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. 2012 Sep 19;7(9):e45252. doi: 10.1371/journal.pone.0045252

Figure 3. Small changes in fragment length or sequence are sensitively detected by HRMA.

Figure 3

(A) Agarose gel electrophoretic analysis of products derived from amplification of SSLP marker microsatellite D7Mit69 from 5 inbred strains of mice. The predicted allele sizes are: FVB; 230 bp, BL/6 and BALB/b; 236 bp, C3H and 129; 238 bp. (B) High Resolution Melt Analysis of the same products showing that the three allele sizes are readily discriminated using either Shifted melting curve or Difference curve analysis. Blue: FVB, Red: C3H and 129, Grey: BALB/b and BL/6. SM: 1 Kb+ DNA ladder, NTC: No template control.