Table 1. Assay conversion.
| Assay | Amplicon Sizes (bp) | No Optimisation | Δ Primer Ratio | Preferred Analysis Method |
| Two-Primer Assays | ||||
| D6Mit268 | 110, 123 | +++ | NT | NMP |
| D6Mit235 | 180, 196 | +++ | NT | NMP |
| D6Mit213 | 116, 150 | +++ | NT | NMP |
| D6Mit201 | 106, 148 | +++ | NT | NMP |
| D6Mit83 | 130, 150 | +++ | NT | NMP |
| D6Mit104 | 146, 158 | +++ | NT | FDC |
| D7Mit76 | 202, 224 | +++ | NT | FDC |
| D7Mit247 | 100, 130 | +++ | NT | NMP |
| D7Mit276 | 114, 132 | + | +++ | NMP |
| D7Mit69 | 232, 238 | + | +++ | FDC |
| D8Mit155 | 139, 151 | +++ | NT | NMP |
| D9Mit89 | 148, 160 | +++ | NT | NMP |
| D9Mit12 | 82, 90 | +++ | NT | FDC |
| D9Mit214 | 116, 138 | +++ | NT | FDC |
| FIC | 437, 536 | +++ | NT | NMP |
| FIT | 400, 453 | +++ | NT | NMP |
| Ube1 sex assay | 132, 138 | +++ | NT | NMP |
| Three-Primer Assays | ||||
| FIN | 278, 400 | +++ | NT | NMP |
| RRF | 279, 383 | +++ | NT | NMP |
| RRFU | 279, 334 | +++ | NT | NMP |
| NLZ | 280, 700 | − | − | FDC |
| ND6 | 240, 470 | +++ | NT | FDC |
| Z5N | 107, 130 | +++ | NT | NMP |
| Four-Primer Assays | ||||
| Mnet | 249, 450 | +++ | NT | NMP |
| K5C | 249, 520 | +++ | NT | NMP |
| K5S | 249, 400 | +++ | NT | NMP |
Amplicon sizes are approximate. Assays were performed in the HRMA reaction conditions and examined by gel electrophoresis and scored as follows: +++; both amplicons produced in equivalent amounts, +; both amplicons produced but one is favoured, −; one or both of the amplicons is not produced. Assays that did not score +++ were optimized with altered primer ratios and again assessed by electrophoresis. NT: not tested, NMP: Normalized melting peak, FDC: Fluorescence difference curve.