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. Author manuscript; available in PMC: 2013 Nov 5.
Published in final edited form as: Mol Cell Endocrinol. 2012 Aug 2;363(1-2):92–99. doi: 10.1016/j.mce.2012.07.012

Fig. 3.

Fig. 3

Sequences between −1636 and −1571, and between −1800 and −1780, are involved in AR-mediated repression of GnRH-E1. GT1-7 cells were transiently transfected with the AR expression plasmid and either the (A), 3’ truncations or (B), 5’ truncations of the GnRH-E1 reporter plasmid on RSVp. Cells were treated with 100 nM R1881 (closed bars) or ethanol vehicle (open bars) for 24 h and subjected to luciferase assay. Data are shown as relative luciferase units, relative to vehicle-treated GnRH-E1, and represent the mean, ± SEM, of at least three experiments done in quadruplicate. Numbers above each set of bars represent percent repression by R1881. *P<0.05, **P<0.01 and *** P<0.001 versus vehicle-treated. (C), Sequence and relative locations of known transcription factor binding sites in GnRH-E1. Box: putative HRE; overline: TPA-responsive site; underline: known transcription factor binding sites.