Table 1.
List of primary antibodies, their sources and dilutions
| Antigen | Source/Cat. # | Clone type* | Dilution** |
|---|---|---|---|
|
| |||
| AcH3K9 | Active Motif/39138 | pAb | 3500 |
| AIB1/SR3 | Active Motif/39798 | pAb | 2500 |
| ATM | Santa Cruz Biotech/23921 | mAb | 750 |
| ATR | Cell Signaling/2790 | pAb | 2500 |
| Caspase 3 | Cell Signaling/9661 | pAb | 1500 |
| CHK1 | Cell Signaling/2345 | pAb | 2000 |
| CHK2 | Cell Signlaing/2662 | pAb | 2500 |
| Cyclin E | Cell Signaling/4129 | mAb | 3000 |
| DNMT1 | Santa Cruz Biotech/10222 | pAb | 700 |
| DNMT3A | Cell Signaling/3598 | mAb | 3000 |
| DNMT3B | Santa Cruz Biotech/10236 | pAb | 700 |
| ELP3 | Active Motif/39950 | pAb | 3000 |
| HDAC4 | Cell Signaling/5392 | mAb | 3000 |
| HDAC5 | Cell Signaling/2083 | pAb | 2500 |
| KAP1 | Bethyl Laboratories A300–774A | pAb | 3000 |
| p53 | Santa Cruz Biotech/126 | mAb | 1000 |
| Poly(ADP-ribose) | Alexis Biochemicals | mAb | 3000 |
| PARP1 | Santa Cruz Biotech/8007 | mAb | 1000 |
| P-ATM (Ser1981) | Rockland/200-301-4000 | mAb | 2000 |
| P-ATR (Ser428) | Cell Signaling/2853 | pAb | 2500 |
| P-CHK1 (Ser317) | Cell Signaling/2344 | pAb | 2500 |
| P-CHK2 (Ser19) | Cell Signaling/2666 | pAb | 2500 |
| P-KAP1 (Ser824) | Bethyl Laboratories A300–767A | pAb | 1500 |
| P-p53 (Ser15) | Cell Signaling/9284 | pAb | 2000 |
| P-SMC1 (Ser957) | Novus Biolog/NB100–205 | pAb | 2000 |
| P-SMC1 (Ser966) | Abcam/1276 | pAb | 3000 |
| SMC1 | Cell Signaling/4802 | pAb | 2500 |
| SRC1 | Cell Signaling/2191 | mAb | 2500 |
| XIAP | Cell Signaling/2045 | mAb | 2000 |
| β-ACTIN | Sigma/A5316 | mAb | 10000 |
| γ-H2AX | Upstate Biotech/05–636 | mAb | 3000 |
| 3MeH3K27 | Active Motif/39155 | pAb | 3500 |
*
pAb: polyclonal antibody; used anti-rabbit IgG (or anti-goat as indicated) for secondary antibody from Bio-Rad Lab
mAb: monoclonal antibody; used anti-mouse IgG for secondary antibody from Bio-Rad Lab
**
Fold dilution in PBS with 0.05% Tween 20