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. Author manuscript; available in PMC: 2013 Sep 19.
Published in final edited form as: Bioconjug Chem. 2012 Aug 30;23(9):1783–1793. doi: 10.1021/bc300036z

Figure 5.

Figure 5

Fluorescence microscopy images of propidium iodide (500 nm) permeable (A), Alexa Fluor 647-annexin V-bound (B) and 3-labeled (C) RGC-5 cells following a 1 hour exposure to 10 µM ionomycin. Bright-field microscopy image of ionomycin-treated RGC-5 cells (D). Overlay of panels A–C showing KcapQ488 (pseudocolor green) activation and co-localization with propidium iodide (pseudocolor blue) and Alexa Fluor 647-annexin V (pseudocolor red) stained cells (E). Overlay of panels D and E (F) (50 µm scale bar).