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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Exp Hematol. 2012 Jun 19;40(10):837–846. doi: 10.1016/j.exphem.2012.06.007

Fig. 4. Endoglin overexpression stimulates primitive hematopoiesis via BMP signaling pathway.

Fig. 4

iEng ES cells were differentiated into EBs for 4.5 days. Dox was added to the culture medium from day 2, whereas Dorsomorphin was added to the culture medium at day 3.5 of EB differentiation. Cells were characterized at day 4.5 as follows: (A) Western blot for the BMP downstream effector, Smad1 and its phosphorylated form Smad1/5/8 (pSmad1/5/8). GAPDH was used as loading control. (B) Quantification of phosphorylated Smad1. After normalization to GAPDH levels, results were plotted as ratio between phosphorylated Smad1/5/8 and total Smad1. Error bars indicate standard errors from 3 independent experiments. (C) Respective iEng EB cultures (± dox; ±dorsomorphin) were assayed for primitive erythroid development. Error bars indicate standard errors from 3 independent experiments. *p<0.05, **p<0.01.