Skip to main content
. Author manuscript; available in PMC: 2013 Oct 21.
Published in final edited form as: Lab Chip. 2012 Oct 21;12(20):3882–3890. doi: 10.1039/c2lc40455a

Fig_5.

Fig_5

(a) Sensorgram showing SLB formation, BSA blocking and CTX-b binding to GM1. 1) vesicle injection; 2) washing excess vesicles from surface; 3) BSA blocking; 4) wash excess BSA; 5) inject CTX-b; 6) end CTX-b injection and begin dissociation step by washing the channel with PBS. (b) Binding kinetics curves of 50 nM CTX-b with SLBs containing various concentration of GM1. The kinetic curves clearly show fast association and a slow dissociation of CTX-b binding to GM1. The maximum spectral shifts increase proportionally with the amount of GM1 receptor up to 2 mol % concentration.