Figure 2a:

(a, b) Scatchard plots show results of a single-binding experiment analyzed with LIGAND software (25), which separated the specific- (▲) and nonspecific-bound (△) radioactivity prior to estimation of the dissociation constant. (a) Mean dissociation constant was 0.36 nmol/L ± 0.14 at 4°C (negative slope of straight solid line). (b) Expanded view of the specific-bound component used to calculate the dissociation constant of the individual assay. Mean dissociation constant of five Cy7 tilmanocept assays was 0.25 nmol/L ± 0.10, which was not significantly different from tilmanocept without Cy7 attached (0.42 nmol/L ± 0.31).