Leukocyte profiles during SIV and MRSA infection. BAL was collected from sham (n = 4) MRSA only, and dual-infected animals 48 h after MRSA infection (n = 4). The black line includes positive cells from SIV-infected animals with and without secondary infection (error bars indicate ± standard error). Treatment is indicated on the X-axis, and positive cells are plotted on the Y-axis. Cells were labeled with antibodies in Mix 1: (a) CD4+, (b) CD8+, (c) 2B11+, and (d) CD21+ or antibodies in Mix 2: (e) CD80+, (f) CD14+, and (g) MHCII+. Only cells within the lymphocyte gate are shown. There was an overall effect of treatment, with a P value of <0.05 for CD4+, 2B11+, CD21+ cells and CD80+ cells. Asterisks indicate a significant difference due to S. aureus treatment (P value <0.05). All other statistical information indicated difference due to SIV infection only.