Table 1.

Diagnostic results of H5N1 influenza A viruses from crop, intestine, and feces and vomit matters of a blow fly after experimental exposure to the virus solution (from [10]).

Postexposure		Replicate	Crop			Intestine			Feces and vomit matters			Virus-exposed cotton
			VI	TCID50	PCRs	VI	TCID50	PCRs	VI	TCID50	PCRs	VI	TCID50 a	PCRs
20ºC		1	+	3.50	+	+	3.50	+
		2	+	3.33	+	+	4.00	+
		3	+	1.83	+	+	0.50	+
		4	+	4.60	+	+	3.50	+
	3 h	5	+	2.60	+	+	3.00	+				NT	5.00	+
		6	+	1.67	+	+	1.67	+
		7	+	<0.50	+	+	<0.50	+
		8	+	4.63	+	+	3.50	+
		9	+	3.00	+	+	3.63	+
		10	+	<0.50	+	+	<0.50	+
		1	+	<0.50	+	+	3.50	+	−	NT	+
	6 h	2	+	<0.50	+	+	1.50	+	−	NT	+	NT	4.50	+
		3	−	<0.50	+	−	<0.50	+	−	NT	+
		1	+	1.00	+	+	1.00	+	−	NT	+
	9 h	2	+	<0.50	+	+	<0.50	+	−	NT	+	NT	5.60	+
		3	−	<0.50	+	−	<0.50	+	−	NT	+
		1	−	<0.50	+	−	<0.50	+	−	NT	+
	24 h	2	−	<0.50	+	−	<0.50	+	−	NT	+	NT	4.67	+
		3	+	1.67	+	+	<0.50	+	−	NT	+
		1	−	NT	+	−	NT	+	+	<0.50	+
	48 h	2	−	NT	+	−	NT	+	−	NT	+	NT	4.83	+
		3	−	NT	+	−	NT	+	−	NT	+
		1	−	NT	+	−	NT	+	−	NT	+
	14 d	2	−	NT	+	−	NT	+	−	NT	+	NT	<0.50	+
		3	−	NT	+	−	NT	+	−	NT	+
		1	−	NT	+	−	NT	+	−	NT	+
10ºC	14 d	2	−	NT	+	−	NT	+	−	NT	+	NT	<0.50	+
		3	−	NT	+	−	NT	+	−	NT	+

VI: virus isolation using embryonated chicken eggs, TCID₅₀: virus titers (log₁₀ TCID₅₀/0.05 mL) were calculated by the inoculation onto MDCK cells,

PCRs: RT-PCR performed with specific primers for the HA and M genes and followed by a nested PCR with primers for the HA gene, NT: not tested.

^aThe average of two times of the virus titration.