Table 2.
Summary of rabbit immunogenicity studies with different EV71 vaccine candidates.
| EV71 vaccine candidates | Adjuvant formulated | Total IgG titers | Western blot | Virus neutralization titer | ||||
|---|---|---|---|---|---|---|---|---|
| VP1-43 | VP2-28 | rVP1 | rVP2 | rVP3 | EV71 (B4 E59) | CVA16 (N5079) | ||
| 2 μg of inactivated | ||||||||
| EV71 virion | PBS | 1/200 | 1/800 | +++ | +++ | ++ | 1/128 | <1/8 |
| EV71 virion | Alum | 1/200 | 1/6400 | +++ | +++ | ++ | 1/6400 | 1/32 |
|
| ||||||||
| 5 μg of inactivated | ||||||||
| EV71 virion | Alum | 1/200 | 1/6400 | +++ | +++ | +++ | 1/12800 | 1/64 |
Two to three rabbits per group were immunized 3 times with EV71 vaccine candidates alone or formulated with alum. The protocols for IgG titer determination, western blot analysis, and virus neutralization assay are described in Section 2.