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. 2012 Sep 12;2012:735132. doi: 10.1155/2012/735132

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Copyright © 2012 David John Tooth et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The distribution of cytosolic proteins in reversed-phase chromatographic fractions of 0.15 M (a), 0.25 M (b), 0.4 M (c), and 1.0 M (d) potassium chloride anion-exchange fractions, derived from a single DDF extract of cultured human Neuro-2A cells. Proteins were quantitated using a BCA method and quantities were normalised as a percentage of total extracted protein (top). Samples of all fractions were analysed using SDS-PAGE stained with Coomassie Blue G-250 (bottom) and total cytosolic extract is also shown (1) for comparison. It is evident that proteins from each of the anion-exchange fractions eluted over a broad range of hydrophobicity using the reversed-phase protocol and were therefore further fractionated.