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Cytotoxicity assays in HEK293 cells using a WST-1 cell proliferation assay. (A) 25K cells plated at 24 h when treated with 10 μM 4 or 5 and DMSO. (B) 25K cells plated at 48 h when treated with 10 μM 4 or 5 and DMSO. Each point is a single biological replicate (N = 3) with 4 technical replicates per biological replicate.
