Preventive
effects of Congo red against Wt-Aβ42 or E22P-Aβ42-induced
neurotoxicity and intracellular ROS accumulation. (A, B) In MTT test,
cultures were treated with Congo red and (A) Wt-Aβ42 (20 μM)
or (B) E22P-Aβ42 (5 μM) for 48 h. (C, D) In DCF assay,
cultures were incubated with Congo red and (C) Wt-Aβ42 (20 μM)
or (D) E22P-Aβ42 (5 μM) for 24 h. The intrinsic fluorescence
of Congo red was subtracted: (∗∗) P < 0.01, (∗∗∗) P < 0.001 vs vehicle, (#) P < 0.05, (###) P < 0.001.