Figure 5.

Effects of KP peptides on IAPP toxicity in pancreatic islet cultures. The effects of 10 μM KP 45–54 (closed red circles) on the toxicity of 5 μM IAPP 1–37 was tested in human pancreatic islet cell cultures (A), with cell viability determined by the MTT assay. The effects of 10 μM KP receptor antagonist P234 or 10 μM NPFF receptor antagonist RF9 on 2.5 μM KP 45–54 protection against the toxicity of 5 μM IAPP 1–37 (B) was tested in human pancreatic islet cell cultures, with cell viability determined by the MTT assay. All results are expressed as % control (pancreatic islet cells in media alone) and are expressed as the mean ± SEM (n = 8). (* = P < 0.05 vs IAPP alone; † = P < 0.05 vs IAPP and KP 45–54; one-way ANOVA.)