Figure 8.

Effects of KP on Congo red binding to Aβ, PrP, and IAPP. The Aβ 1–40, Aβ 1–28, Aβ 25–35, Aβ 29–40, PrP 106–126, PrP 118–135, IAPP 1–37, IAPP 20–29, A-Bri 1–34, and A-Dan 1–34 peptides were dissolved in PBS and incubated at 37 °C for 24 h, with constant oscillation, with or without KP 45–50. Congo red binding assays (A) were performed with amyloid peptides alone (open columns) or in the presence of KP 45–50 (closed blue columns). The effect of Congo red (0–100 mM) on biotinylated Aβ 1–42 binding to KP 45–54 (B) was determined by EIA. All results are expressed as the mean ± SEM (n = 8). (* = P < 0.05 vs amyloid fibrils alone; one-way ANOVA.)