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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Curr Protoc Chem Biol. 2012 Jun 1;4(2):110236. doi: 10.1002/9780470559277.ch110236

Figure 2. Schematic describing the operation of the breeding vessel (iSpawn).

Figure 2

(a) Insert the mesh spawning platform into the vessel and fill up the vessel with water. Place male fish above the spawning platform. (b) Place mesh separator into the vessel so male fish are confined in the bottom half of the iSpawn. (c) Add in female fish above the separator. Allow fish to prime overnight. (d) On the next morning, remove the separator so that male and female fish mix in the deep water. (e) Raise the spawning platform so that fish are in shallow water. Allow fish to spawn for desired time (around 10 minutes). (f) Remove the spawning platform and fish to stop spawning. Place a strainer at the bottom of the vessel. Open the valve and drain the water into the strainer. Collect the fallen embryos.