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(a) Measured phase shift during a hypo-osmotic shock using off-axis phase holography on two HEK293 cells. After initial rapid cell swelling, active cell volume regulation is observed. (b) Measured phase shift during a similar hypo-osmotic shock as in (a), but with Zinc added to the extracellular solution. The Zn2+-ions inhibit Cl- channels in the cell membrane, resulting in an absence of active volume regulation.
