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(a) THG images of spleen extract. White arrows indicate hollow-core splenocytes. (b-d) The (b) TPF, (c) THG, and (d) combined images of an anti-D3ε-Allophycocyanin labeled T-lymphocyte. Scale bar: 5 μm. (e) The bright-field image of lymphocyte with Wright-Giemsa stain. Scale bar: 5 μm (f) The THG images of hollow-core leukocytes without anti-CD3ε-APC targeting in spleen extracts. Scale bar: 10 μm.
