Figure 6.

IBP knockdown results in increased cisplatin-mediated apoptotic cell death. (A) After a 24 h cisplatin treatment, the cell lysates of the MCF-7/IBP-RNAi cells and the control cells were prepared, and the cytoplasmic marker of apoptosis (cleaved PARP) was detected by western blot. (B) MCF-7/IBP-RNAi cells and the control cells were treated with 2 μg/ml cisplatin to induce apoptosis. The apoptosis was detected at 12 h after cisplatin treatment by Alexa fluor 647 conjugated Annexin V-PI assays.