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. 2012 Jul 7;13:36. doi: 10.1186/1471-2172-13-36

Table 2.

Sequences of primer for quantitative RT-PCR of microRNA

MicroRNAs Primer sequence
miR-181a
Forward: 5′-AACATTCAACGCTGTCGGTG-3′
miR-155
Forward: 5′-GGGGTTAATGCTAATTGTGATAGG-3′
miR-150
Forward: 5′-TCTCCCAACCCTTGTACCA-3′
miR-146a
Forward: 5′-GGGTGAGAACTGAATTCCATGG-3′
miR-125b
Forward: 5′-TCCCTGAGACCCTAACTTGTGA-3′
Universal reverse primer
Reverse primer: 5′-GCGAGCACAGAATTAATACGACTC-3′
Reverse transcription primer Poly (T) adapter: 5′-GCGAGCACAGAATTAATACGACTCACTATAGG (T)12VN-3′ (V = A,G,C; N = A,T,G,C) [Virginie Olive 2009]