Figure 2.

TR4^−/−bone marrow cultures have reduced osteoblast differentiation. Bone marrow cells were isolated from 10 weeks old TR4^−/− and control TR4^+/+ mice. Cells were cultured in 2 mL α-MEM containing 10% FBS at 5x10⁶ cells/well in 6-well plates. After 7 days, the media was replaced with media containing β-glycerophosphate and ascorbic acid to induce osteoblast differentiation. Colonies were stained on day 21 after plating by alkaline phosphatase (A) and alizarin red ( B). The assays were performed in triplicate.