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. 2012 Sep 20;8(9):e1002935. doi: 10.1371/journal.ppat.1002935

Figure 2. Infection of different cell types by gp150+ and gp150− MuHV-4.

Figure 2

a. HS+ and HS− CHO-K1 cells were exposed to gp150+ and gp150− versions of EF1α-eGFP MuHV-4 for 18 h. 20,000 cells were then assayed for viral eGFP expression by flow cytometry. Equivalent protein content of the gp150+ and gp150− virus stocks was confirmed by Coomassie staining (Fig. S2). b. RAW-264, NS0 and A20 cells were infected and analysed as in a. Bars show mean ± SD of 3 replicate experiments. Gp150− virions infected RAW-264 and NS0 cells significantly better than did gp150+ virions (p<0.01 by Student's 2-tailed t test).