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. 2012 Sep 20;8(9):e1002925. doi: 10.1371/journal.ppat.1002925

Figure 2. The effect of Relacin on Rel-ribosomes interaction.

Figure 2

(A) Relacin inhibits dissociation of Rel/Spo from the ribosome. The relative amount of Rel/Spo (D. radiodurans) bound to purified ribosomes was quantified following the addition of increasing levels of Relacin. Rel/Spo molecules associated with 70S complexes were detected by Western blot analysis (see Materials and Methods). Histogram indicates the average of two independent biological repeats. Error bars represent the range. (B) Ribosome independent inhibition of (p)ppGpp synthesis. The constitutively active, ribosome-independent RelAC638F (E. coli) protein was treated with increasing concentrations of Relacin, as indicated (see Materials and Methods) in the presence or absence of isolated ribosomes. Shown is the average of duplicates of a representative experiment. Error bars represent the range.