Table 1. Image reconstruction data.
Cp183e-SSS | Cp183e-EEE | Cp183RNA-SSS | Cp183RNA-EEE | |
Number of particles (in reconstruction/total) | 27489/36676 | 14416/16967 | 7201/10502 | 7439/9196 |
Number of CCD frames | 594 | 294 | 193 | 394 |
Nominal magnification | 80,000× | 80,000× | 40,000× | 80,000× |
Pixel size (Å) | 1.484 | 1.484 | 2.940 | 1.484 |
Defocus ranges (µm) | 0.16–4.10 | 0.60–3.13 | 0.27–3.80 | 0.54–4.87 |
Resolution (Å) | 5.5 | 5.8 | 8.0 | 7.0 |
Resolution was estimated based on a Fourier Shell Correlation of 0.5.