Figure 7. GAGE proteins bind dsDNA.

A–B. Western blot of pulldown experiments testing the binding of recombinant GAGE12I (A) or endogenous GAGE proteins from MZ2-MEL lysates (B) to cellulose alone, or cellulose-immobilized native calf thymus dsDNA. C. Electrophoretic mobility shift assays. Each indicated concentration of purified GAGE12I was incubated with the ³²P-labelled 90-bp EcoRI-PvuII DNA fragment from pUC19 at 1 pg/µl; samples were then resolved by agarose gel electrophoresis. D. Same experiment as in (C), but with addition of NaCl. E. Prediction of putative DNA binding amino acids in GAGE12I using the program BindN. +/− = binding/no binding; 0–9 = confidence of binding prediction.