Table 1. Order of treatments.

study day	animal 1	animal 2	animal 3	animal 4	animal 5
1	3 mg/kg	vehicle	1 mg/kg	3 mg/kg	vehicle
2	vehicle	6 mg/kg	10 mg/kg	vehicle	3 mg/kg
3	6 mg/kg	3 mg/kg	vehicle	6 mg/kg	6 mg/kg
4	10 mg/kg	1 mg/kg	3 mg/kg	10 mg/kg	10 mg/kg
5	1 mg/kg	10 mg/kg	6 mg/kg	1 mg/kg	1 mg/kg

Table 1 shows the randomised Latin square design employed in the current study. Such a design allows all subjects to receive all treatments, once, in a different, randomly assigned, order, thereby minimising the risk of carryover effect.