Table 1. Thermodynamic parameters for the binding of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) to WspC-Pp and its shortened versions WspC-Ppmiddle (WspC-Pp lacking TPR domain) and WspC-Ppshort (WspC-Pp lacking TPR domain and linker).

Ligand 1	Ligand 2	K D (µM)	K A (M−1)	ΔH kcal/mol	TΔS kcal/mol
WspC-Pp	SAM	43±2	(2.3±0.1) 104	−7.43±0.6	−1.49±0.6
WspC-Ppmiddle	SAM	35±2	(2.9±0.1) 104	−3.04±0.4	3.04±0.4
WspC-Ppshort	SAM	41±4	(2.5±0.2) 104	−3.53±0.4	2.46±0.5
WspC-Pp	SAH	1.7±0.1	(5.9±0.1) 105	−21.7±0.1	−13.8±0.1
WspC-Ppmiddle	SAH	1.6±0.1	(6.3±0.2) 105	−14.8±0.2	−6.88±0.2
WspC-Ppshort	SAH	2.0±0.1	(4.9±0.1) 105	−18.6±0.2	−10.8±0.2