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. 2012 Sep 20;7(9):e45810. doi: 10.1371/journal.pone.0045810

Table 3. Oligonucleotides used in this study.

Name sequence Construction of
WspC-Pp-f 5′-AGGGATGCCCATATGAACGAACAGCGTTTC-3′ pET28b-WspC-Pp
WspC-Pp-r 5′-TCGCGAATTGGATCCTCATCGTTCAGACTC-3′ pET28b-WspC-Pp
WspC-Ppshort-f 5′-AGGGATGCCCATATGAACGAACAGCGTTTC-3′ pET28b-WspC-Ppshort
WspC-Ppshort-r 5′-TTGGCAGTGGATCCTCATGCCGCCGCCAA-3′ pET28b-WspC-Ppshort
WspC-Ppmiddle-f 5′-AGGGATGCCCATATGAACGAACAGCGTTTC-3′ pET28b-WspC-Ppmiddle
WspC-Ppmiddle-r 5′-AGTAGGATCCTCATGCCGACGGCGGGTACT-3′ pET28b-WspC-Ppmiddle