Table 3. Oligonucleotides used in this study.
Name | sequence | Construction of |
WspC-Pp-f | 5′-AGGGATGCCCATATGAACGAACAGCGTTTC-3′ | pET28b-WspC-Pp |
WspC-Pp-r | 5′-TCGCGAATTGGATCCTCATCGTTCAGACTC-3′ | pET28b-WspC-Pp |
WspC-Ppshort-f | 5′-AGGGATGCCCATATGAACGAACAGCGTTTC-3′ | pET28b-WspC-Ppshort |
WspC-Ppshort-r | 5′-TTGGCAGTGGATCCTCATGCCGCCGCCAA-3′ | pET28b-WspC-Ppshort |
WspC-Ppmiddle-f | 5′-AGGGATGCCCATATGAACGAACAGCGTTTC-3′ | pET28b-WspC-Ppmiddle |
WspC-Ppmiddle-r | 5′-AGTAGGATCCTCATGCCGACGGCGGGTACT-3′ | pET28b-WspC-Ppmiddle |