Table 2.
RNA sequencing cytoskeletal gene expression
| Gene | FPKM |
||||
|---|---|---|---|---|---|
| WT | SRF cKO | MKL1 KO | MKL2 cKO | DKO | |
| Actb | 7795.17 | 2219.60* | 2696.76* | 5856.10 | 918.85* |
| Cfl1 | 1002.17 | 620.35 | 548.28 | 690.11 | 723.96 |
| Cnn2 | 247.85 | 102.00* | 42.33* | 220.86 | 41.44* |
| Flna | 839.82 | 304.74* | 309.33 | 583.87 | 56.52* |
| Myh9 | 999.37 | 631.94 | 1017.94 | 1311.00 | 107.44* |
| Pfn1 | 1935.96 | 918.24 | 1014.16 | 1359.71 | 1396.58 |
| Tpm2 | 1.04 | 1.02 | 0.54 | 1.03 | 4.08 |
| Tpm4 | 503.25 | 172.53* | 259.49 | 510.30 | 53.64* |
| Vcl | 201.99 | 65.07* | 99.46 | 171.20 | 12.10* |
RNA sequencing shows different expression levels of genes important in maintaining and remodeling the actin cytoskeleton. Reads represent fragments per kilobase per million and fold changes indicate log2 differences. Data are displayed in FPKM.
FPKM indicates fragments per kilobase of transcript per million; WT, wild type; SRF, serum response factor; KO, knockout; cKO, conditional knockout; and DKO, double knockout.
*
Differentially expressed genes reaching statistical significance (q < 0.05) compared to WT.