|
pAK23
|
pMin1, upstream region of comB6, downstream region of comB10,sepa-rated by aphA-3 cassette, TetR, ErmR
|
[10]
|
|
pBluescript II SK+
|
ori
colE1, ori
f1(+), lacZ, M13 forward−/reverse primer binding sites, AmpR
|
Stratagene |
|
pCR2.1-TOPO
|
ori
colE1, ori
f1, lacZ, M13 forward-, reverse- and T7 promotor/primer binding sites,AmpR, KanR
|
Invitrogen |
|
pDH29
|
pBluescript II KS+
recA::erm, AmpR, ErmR
|
[40]
|
|
pJP44 (
rpsL-erm
)
|
ΔcagPAI::rpsL-erm
|
This study |
|
pSR11
|
pBluescript IISK rpsL-erm-cassette in BamHI |
This study |
|
pSR12
|
Deletion of T4SS tfs3; rpsL-erm
|
This study |
|
pSR13
|
Deletion of T4SS tfs3 rpsL-erm deleted |
This study |
|
pSR14
|
Deletion of T4SS tfs4; rpsL-erm
|
This study |
|
pSR15
|
Deletion of T4SS tfs4; rpsL-erm deleted
|
This study |
|
pSR18
|
Deletion of T4SS of cagPAI; rpsL-erm (pJP44+rpsL-erm) |
This study |
|
pSR19
|
Deletion of T4SS comB; rpsL-erm (pAK23+rpsL-erm) |
This study |
|
pSR20
|
pSP76 carrying aphA-3 cassette; in moeB locus |
This study |
|
pSR21
|
Deletion of virB4/topA; Insertion of rpsL-erm cassette |
This study |
|
pSR23
|
Insertion of virB4/topA [ATCC43526] |
This study |
|
pSR24
|
Deletion of orf12GH in pHel12; Insertion of aphA-3-cassette |
This study |
|
pSR25
|
Deletion of orf12GH in pHel12; Insertion of catGC-cassette |
This study |
|
pSR26
|
Deletion of orf12C in pHel12; Insertion of catGC cassette |
This study |
|
pSR27
|
Deletion of orf12C in pHel12; Insertion of aphA-3-cassette |
This study |
|
pSR28
|
Insertion of catGC cassette between orf4M and orf4A in pHel12 |
This study |
|
pSR29
|
Deletion of tfs3 including chromosomal relaxase using rpsL-erm
|
This study |
|
pSR33
|
Deletion of comE3; Insertion of aphA-3-cassette |
This study |
|
pWS48
|
pBluescript II KS+ carrying recA (partial)::cat, AmpR, CamR
|
[40]
|
|
pWS102
|
Deletion of vacA; Insertion of aphA-3-cassette |
This study |
