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. 2012 Sep 20;7(9):e45561. doi: 10.1371/journal.pone.0045561

Figure 1. Stathmin is efficiently knocked out in C57BL/6 and FVB mouse strains.

Figure 1

(A) PCR analyses of genomic DNA extracted from the tail of WT, stathmin heterozygous and KO C57BL/6 and FVB mice. (B) qRT-PCR analyses of stathmin mRNA expression in brain and thymus from 10 weeks old WT and stathmin KO C57BL/6 and FVB mice. (C) qRT-PCR analyses of stathmin mRNA expression in bladder and skeletal muscle from 10 weeks old WT and stathmin KO C57BL/6 mice. (D) and (E) Western Blot analysis of stathmin protein expression in thymus and spleen of 15 weeks old WT and stathmin KO C57BL/6 mice (D) and in mouse embryo fibroblasts (MEF) isolated from 13.5 days old C57BL/6 or FVB embryos (E). Vinculin was used as loading control.