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. 2012 Sep 20;7(9):e45922. doi: 10.1371/journal.pone.0045922

Figure 1. Purification of PFL.

Figure 1

(A) Gel filtration on a Superose 12 column. An aliquot of cell lysate from E. coli expressing PFL was subjected to Superose 12 column equilibrated with 0.15 M NaCl in 20 mM phosphate buffer (PBS). The column was eluted with PBS at a flow rate of 0.8 ml/min by an isocratic mode. The eluate was monitored by absorption at 280 nm and examined for hemagglutination activity, and the active fractions denoted by bar were pooled. (B) SDS-PAGE of purified PFL. The purified PFL was applied on 10% PAGE under reducing conditions. Lane 1, molecular weight standards; Lane 2, purified PFL.