Figure 9. Involvement of high mannose glycans on PFL-integrin α2 interaction.

(A) Effect of yeast mannan (YM) on PFL-induced internalization of integrin α2 was determined by immunofluorescence using a fluorescence microscope. MKN 28 cells were incubated with 20 μg/ml Alexa488-PFL in RPMI for 4 h in the presence or absence of 700 μg/ml yeast mannan (YM). The cells were fixed with 80% acetone and visualized with mouse monoclonal anti-integrin α2/CD49b antibody followed by with Alexa568-conjugated 2nd antibody. (B) Effect of various lectins on cell surface integrin α2. MKN 28 cells were incubated with 20 μg/ml of various lectins with different specificity in RPMI for 4 h. The cells were visualized using an anti-integrin α2/CD49b antibody as above.