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. 2012 Sep 20;7(9):e45844. doi: 10.1371/journal.pone.0045844

Figure 5. Identification of RE-1-containing genes potentially involved in beta cell survival.

Figure 5

A. Alignment of identified RE-1 sequences in human (hum) or murine (mus) genes with the consensus RE-1. Left alignment identifies members of the insulin-like growth factor transduction pathway: Igfbp7: insulin-like growth factor binding protein 7; IRS: insulin receptor substrate; Akt3: PKB gamma; CTNND2: delta2 catenin; Ctnnb1: beta catenin; MAPK10: JNK3; MAP4K3: MEK kinase kinase 3. Upper right alignment identifies members of the cyclin-related family of mitogenic factors: Cdk5r2: cyclin-dependent kinase 5 regulatory subunit 2; CKS2: CDC28 protein kinase regulatory subunit 2; CNNG1: cyclin G1; Cnnm2: cyclin M2. Lower right alignment identifies Api5: apoptosis inhibitor 5 and Nrg1: neuregulin1. B. Quantitative RT-PCR analysis of mRNA levels from control INS-1E cells (white bars) and INS-1E cells infected with GFP-expressing adenovirus (gray bars) or REST-expressing adenovirus (black bars). Ptprn: protein tyrosine phosphatase, receptor type, N; Irs2: insulin receptor substrate2; Cdk5r2: cyclin-dependent kinase 5 regulatory subunit 2; Nrg1: neuregulin1; Ctnnd2: delta2 catenin; Api5: apoptosis inhibitor 5. Results are mean ± SD of six independent experiments. *P<0.05, **P<0.01, ***P<0.001 versus INS-1E cells transduced with GFP. C. Quantitative RT-PCR analysis of mRNA levels from islets of WT (white bars) (n = 5) and RIP-REST (black bars) (n = 5) mice. Gjd2: Connexin36; Mapk8ip1: Ib1; Cdk5r2: cyclin-dependent kinase 5 regulatory subunit 2; Ptprn: protein tyrosine phosphatase, receptor type, N; Irs2: insulin receptor substrate2; Nrg1: neuregulin1; Ctnnd2: delta2 catenin. Results are mean ± s.e.m. **P<0.01 versus values of wild type animals. D. Left panel, immunoblotting of total proteins from non infected (NI) and infected (Ad-GFP, Ad-REST) INS-1E cells showing decreased protein levels of Cdk5r2 upon REST expression. The right panel shows the corresponding quantifications of REST protein levels in INS-1E cells after infection. Data are mean ± SEM of 4 independent experiments. **P<0.01, ***P<0.001 versus the respective Ad-GFP-infected and NI conditions.