NP, normal parathyroid tissue; PH, parathyroid hyperplasia PA, parathyroid adenoma; PC, parathyroid carcinoma A–D H&E staining of normal parathyroid tissue,parathyroid hyperplasia, parathyroid adenoma and parathyroid carcinoma E–G IHC of Parafibromin is specific nuclear staining in normal parathyroid tissue,parathyroid hyperplasia, parathyroid adenoma, brown granulation can be seen in positive nuclear side H Typical loss staining of Parafibromin in PCA–D H&E staining of normal parathyroid tissue,parathyroid hyperplasia, parathyroid adenoma and parathyroid carcinoma E–G IHC of Parafibromin is specific nuclear staining in normal parathyroid tissue, parathyroid hyperplasia, parathyroid adenoma, brown granulation can be seen in positive nuclear side H Typical loss staining of Parafibromin in PC.