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. 2012 Sep 20;7(9):e45562. doi: 10.1371/journal.pone.0045562

Figure 5. Glioblastoma cell-secreted IL-8 modulates brain endothelial cell properties.

Figure 5

A–B) IL-8 secretion was decreased in U87 using three independent IL-8-targeting siRNA (si-1, si-2, and si-3) with the non-silencing sequence (SIC) serving as control. IL-8 siRNA efficiency was confirmed by ELISA (A) and RT-PCR (B). C–E) Three day-old starved human cerebral microvascular endothelial cells (hCMEC/D3) were stimulated with U87 conditioned medium (CM, 1/20 diluted) collected 72 h after transfection with IL-8 siRNAs, and analyzed for ERK activation (pERK1/2) by western-blot (C), permeability to FITC-dextran (D), and VE-cadherin (VEC) immunolocalization (E). Ratios between pERK1/2 and ERK2 intensities are indicated below scans. Scale bar: 10 µm. One out of three independent experiments is shown. T test on 3 independent experiments: **p<0.01.