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. 2012 Sep 20;7(9):e45695. doi: 10.1371/journal.pone.0045695

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© 2012 Al-Salih et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A,B) Immunostaining for CD42b/GPIb and for plasminogen, respectively, with hemalun nuclear counterstaining (x10), showing positive immunostaining in the VG. (C) Immunostaining for tissue-type PA (tPA). Insets: higher magnification (x20) of the interface VG/valve (left-hand side) and within the valvular tissue (right-hand side). (D) Immunostaining for urokinase (uPA). Inset: higher magnification (x20) of the interface VG/valve showing an intense staining of polymorphonuclear cells.