Figure 6. Plasmin activity in the conditioned media.

(A) Western blot analysis for detection of plasmin(ogen) with a monoclonal anti-human plasminogen antibody, in conditioned medium obtained from the VG and N samples of 2 representative patients (Pn and Pg: purified human plasmin, 0.25 µg per well, and purified human plasminogen 0.2 µg per well, respectively, used as reference). Positions in the gel of molecular mass standard proteins used for calibration are indicated on the left. (B) Plasmin activity quantified in the conditioned media of N and VG, using a selective chromogenic substrate (P = 0.8).